Abstract: The potato rot nematode, Ditylenchus destructor Thorne, is an important quarantine nematode in China. In order to obtain extracts with high attractions to D. destructor, the chemotaxis of D. destructor to Botrytis cinerea, Fusarium moniliforme, F. semitectum and F. graminearum were investigated on water agar plates. The results indicated that the chemoreceptive response of nematodes varied with different fungi species. The percentage of nematodes that attracted to the living disc and culture filtrates of B. cinerea after 36 h was 15% and 8% respectively, which was significantly higher than those of F. moniliforme, F. semitectum and F. graminearum. The chemotaxis of D. destructor to B. cinerea increased with exposure time, 78 nematodes (26%) accumulated in or under the mycelia discs of B. cinerea after 96 h. The numbers of accumulated nematodes around mycelia discs and those around disc that heated for 120 min at 80 ℃ of B. cinerea were not significantly different, demonstrating that the active compounds has strong heat stability. The attracted percentage of nematodes of the ethanol extract and n-butanol extract were 11.6% and 11.0%, which were significantly higher than those of the other treatments. This research is helpful to develop new strategies of nematode control in sweet potato production.